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Publications

Publications by Pedro Jorge

2020

Colorimetry-based system for gaseous carbon dioxide detection: Membrane optimization

Authors
Mendes, JP; Coelho, L; Pereira, CM; Jorge, PAS;

Publication
U.Porto Journal of Engineering

Abstract
The study of sensing materials to the detection of carbon dioxide (CO2) was achieved using p-nitrophenol (pNPh) as a colorimetric indicator. The sensing material was polymerized (NPLn), functionalized with 3-triethoxysilyl propyl isocyanate (IPTES) which sensitivity was tested in the form of a membrane as is and encapsulated in hollow silica nanoparticles. The sensing membranes were tested in a closed gas system comprising very precise flow controllers to deliver different concentrations of CO2 (vs. N2). The combination of the sensing membranes with multimode optical fibers and a dual-wavelength diode (LED) allows the measurement of the CO2 through the analysis of the induced absorbance changes with a self-referenced ratiometric scheme. The analysis of the sensing materials have shown significant changes in their chemical and physical properties and the results attest these materials with a strong potential for assessing CO2 dynamics in environmental, medical, and industrial applications.

2021

Biosensors for Biogenic Amines: A Review

Authors
Vasconcelos, H; Coelho, LCC; Matias, A; Saraiva, C; Jorge, PAS; de Almeida, JMMM;

Publication
BIOSENSORS-BASEL

Abstract
Biogenic amines (BAs) are well-known biomolecules, mostly for their toxic and carcinogenic effects. Commonly, they are used as an indicator of quality preservation in food and beverages since their presence in higher concentrations is associated with poor quality. With respect to BA's metabolic pathways, time plays a crucial factor in their formation. They are mainly formed by microbial decarboxylation of amino acids, which is closely related to food deterioration, therefore, making them unfit for human consumption. Pathogenic microorganisms grow in food without any noticeable change in odor, appearance, or taste, thus, they can reach toxic concentrations. The present review provides an overview of the most recent literature on BAs with special emphasis on food matrixes, including a description of the typical BA assay formats, along with its general structure, according to the biorecognition elements used (enzymes, nucleic acids, whole cells, and antibodies). The extensive and significant amount of research that has been done to the investigation of biorecognition elements, transducers, and their integration in biosensors, over the years has been reviewed.

2021

Development of a Long Period Fiber Grating Interrogation System Using A Multimode Laser Diode

Authors
Silva, LH; Santos, P; Coelho, LCC; Jorge, P; Baptista, JM;

Publication
SENSORS

Abstract
Optical fiber gratings have long shown their sensing capabilities. One of the main challenges, however, is the interrogation method applied, since typical systems tend to use broadband light sources with optical spectrum analyzers, laser scanning units or CCD (Charged Coupled Device) spectrometers. The following paper presents the development of an interrogation system, which explores the temperature response of a multimode laser diode, in order to interrogate long period fiber gratings. By performing a spectral sweep along one of its rejection bands, a discrete attenuation spectrum is created. Through a curve fitting technique, the original spectrum is restored. The built unit, while presenting a substantially reduced cost compared with typical interrogation systems, is capable of interrogating along a 10 nm window with measurement errors reaching minimum values as low as 0.4 nm, regarding the grating central wavelength, and 0.4 dB for its attenuation. Given its low cost and reduced dimensions, the developed system shows potential for slow-changing field applications.

2021

Effect of Low-Doses of Gamma Radiation on Electric Arc-Induced Long Period Fiber Gratings

Authors
Mesonero Santos, P; Fernandez Medina, A; Coelho, LCC; Viveiros, D; Jorge, PA; Belenguer, T; Heredero, RL;

Publication
SENSORS

Abstract
This work presents an experimental study on the effects of gamma radiation on Long Period Fiber Gratings (LPFGs) in a low-dose test campaign to evaluate their eventual degradation. The study was carried out with standard single-mode fibers where the grating was inscribed using the Electric-Arc Discharge (EAD) technique. Before the gamma campaign, a detailed optical characterization was performed with repeatability tests to verify the accuracy of the setup and the associated error sources. The gamma-induced changes up to a dose of 200 krad and the recovery after radiation were monitored with the Dip Wavelength Shift (DWS). The results show that the gamma sensitivity for a total dose of 200 krad is 11 pm/krad and a total DWS of 2.3 nm has been observed with no linear dependence. Post-radiation study shows that recovery from radiation-induced wavelength shift is nearly complete in about 4000 h. Experimental results show that the changes suffered under gamma irradiation of these LPFGs are temporary making them a good choice as sensors in space applications.

2021

Detection of biogenic amines in several foods with different sample treatments: An overview

Authors
Vasconcelos, H; de Almeida, JMMM; Matias, A; Saraiva, C; Jorge, PAS; Coelho, LCC;

Publication
TRENDS IN FOOD SCIENCE & TECHNOLOGY

Abstract
Background: Biogenic amines (BAs) are compounds considered to be contaminants of foodstuff and are cause of poisoning. The main BAs found in foods are cadaverine, putrescine, tyramine, histamine, spermine and spermidine. The number of food poisoning cases associated with BAs in food has increased in the recent years reinforcing the need for early detection to ensure high levels of food quality and safety. Scope and approach: This review aims to provide a general approach to the different BAs detected in foods their concentrations and sample treatments. These compounds are found in varying concentrations in a wide variety of foods such as fish, meat, fruits, vegetables, cheese, wine, and beer. It also refers the different analytical techniques currently used for the detection of BAs, as well as the different treatments of the samples and innovations of the techniques currently used that allow greater sensitivity and speed of the analyzes and with obtaining detection limits lower and lower. Key findings and conclusions: BAs are present in a wide variety of foods and their concentration is highly influenced by the storage conditions of food products. BAs can be precursors of nitrosamines, which have been linked to carcinogenic and mutagenic activity. Several analytical techniques and sample treatments have been improved in the last few years for better and faster detection of BAs.

2021

Turn Around Point Long Period Fiber Gratings With Coupling to Asymmetric Cladding Modes Fabricated by a Femtosecond Laser and Coated With Titanium Dioxide

Authors
Viveiros, D; de Almeida, JMMM; Coelho, L; Vasconcelos, H; Maia, JM; Amorim, VA; Jorge, PAS; Marques, PVS;

Publication
JOURNAL OF LIGHTWAVE TECHNOLOGY

Abstract
A detailed study of turn around point (TAP) long period fiber gratings (LPFGs) with coupling to the asymmetric cladding modes of a standard single-mode fiber (SMF-28e), fabricated by femtosecond (fs) laser direct writing was realized. The entire fabrication process, including the coating with different titanium dioxide (TiO2) film thicknesses of LPFGs and the corrections needed to achieve coated devices operating precisely in the TAP condition with coupling to the asymmetric cladding modes, was addressed. The significant fabrication details are also given, such as inscription periods, shape and localization of the refractive index modifications across the core. The fabrication process described allows the optimization of the LPFGs sensitivity in regards to the surrounding refractive index (SRI). Optimization of the writing parameters to obtain gratings working at the TAP for two different media surrounding the fiber (water and air) was achieved. It was demonstrated that for a grating period of 191.8 mu m, the LP1,12 mode exhibits a TAP at 1442.7 nm in air, and for a period of 192.5 mu m, the same mode exhibits a TAP at 1448.6 nm in water. The LPFGs operating at the TAP in air and water were coated with 10, 20, and 30 nm thin TiO2 film thicknesses and the spectral behavior characterized. The wavelength sensitivity to the surrounding refractive index (SRI) was assessed in the range between 1.3700 to 1.4120, and a maximum sensitivity of similar to 8051.4 nm/RIU was measured for the 192.5 mu m LPFG coated with a 30 nm thick TiO2 film.

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