Abstract
The application of organic wastes to agricultural soils may have adverse effects on soil quality. Different end-points are proposed to be analysed and included in organic waste management programmes. The aim of this laboratory study was to determine how the application of increasing concentrations of broiler chicken litter, dairy sludge and cattle slurry can affect survival, body mass change and metal accumulation in Eisenia fetida (Savigny, 1826). Generally, no mortality occurred for the different organic wastes concentrations at the end of the study. In addition, significant increases of body mass were observed on earthworms exposed to the highest concentrations of the organic wastes. Organic wastes increased concentration of some metals in soil (mainly, Cu and Zn in broiler litter and dairy sludge mixtures) and in E. fetida tissue (mainly, Cu in earthworms from broiler litter containers), although metal contents in soil-waste mixtures were always below legal limits. The negligible toxic effect of organic wastes on earthworms was due to the low heavy metal levels of those organic wastes and due to some nutritional or habitat advantages promoted by them. Results obtained demonstrated that body mass change was the most sensitive end-point and, therefore, should be integrated on a battery of ecotoxicological assays to evaluate the ecotoxicity of this type of organic wastes.

Abstract
Plantain (Plantago spp.) is generally considered to be an anemophilous plant that produces pollen of low nutritional value to bees. Between April and September during 2002 and 2003, in two regions in northwestern Portugal, we studied both the pollen collected by honey bee colonies and airborne pollen. Plantain pollen collection by honey bees was observed only in three different periods, despite its continuous presence in the air. This behaviour was related to the beginning and ending of flowering of the honey bee's main nutritional pollen sources, with no other factor seemingly influencing the temporal collecting patterns. The collection of Plantago spp. pollen was observed in the gaps between the flowering of Eucalyptus spp., chestnut (Castanea sativa), wild blackberry (Rubus spp.) and heaths (Ericaceae family). The results suggest that pollen of Plantago species seems to be used by honey bees as a substitute for other more nutritious pollen types and is collected only as a supplementary source. The temporal patterns of Plantago pollen collection by honey bees could therefore be used as a valuable ecological indicator of periods with low availability of highly nutritious pollen sources.

Abstract
This paper proposes a method to unify the definition of the main pollen season based on statistical analysis. For this, an aerobiological study was carried out in Porto region (Portugal), from 2003-2005 using a 7-day Hirst-type volumetric spore trap. To define the main pollen season, a non-linear logistic regression model was fitted to the values of the accumulated sum of the daily airborne pollen concentration from several allergological species. An important feature of this method,is that the main pollen season will be characterized by the model parameters calculated. These parameters are identifiable aspects of the flowering phenology, and determine not only the beginning and end of the main pollen season, but are also influenced by the meteorological conditions. The results obtained with the proposed methodology were also compared with two of the most used percentage methods. The logistic model fitted well with the sum of accumulated pollen. The explained variance was always higher than 97%, and the exponential part of the predicted curve was well adjusted to the time when higher atmospheric pollen concentration was sampled. The comparison between the different methods tested showed large divergence in the duration and end dates of the main pollen season of the studied species.

Abstract

Abstract
A forecast model for estimating the annual variation in regional olive crop yield has been developed during the years 1998 to 2006 for the Alentejo region (south Portugal). This model was estimated hierarchically along three different in-season stages: i) flowering, considering only the regional airborne pollen index; ii) fruit growth with the addition of a plant water-stress index; iii) fruit maturing with the addition of a phytopathological index. Appropriated statistical tests indicated that the regional airborne pollen index accounted for 64% of the inter-annual olive yield variability with an average deviation between observed and predicted production of 16%. The addition of the variable plant water-stress index to the forecasting model allowed an increase in its accuracy of 27% while the phytopathological index allowed an increase of 6%. The final bioclimatic model, with all the three variables tested, explained 97% of the regional olive fruit yield being the average deviation between observed and predicted production of 4% for the internal validation of the model and of 9% for the external validation. The hierarchical structure of this bioclimatic model, along three different development stages, enabled an update along the growing season.

Abstract
Pollen morphology and quality of 20 olive cultivars grown in Portugal were analysed and multivariate statistical analysis was performed in order to group the cultivars with similar characteristics. The morphology was analysed by scanning electron microscopy and the quality was evaluated through pollen viability and in vitro germination. Pollen grains from the studied cultivars were elliptical, subprolate or prolate spheroidal with an average size (22x20 mu m), 3- zonocolpate for the majority of cultivars but 3-zonocolporate for three of the cultivars studied. Pollen viability varied between 76% ('Verdeal de Tras-os- Montes') to 6% ('Madural') and the germination varied between 65% ('Verdeal de Tras-os-Montes') and 2% ('Conserva de Elvas'). Hierarchical cluster analysis based on all characteristics analysed, showed an organization into three groups composed by cultivars with similar pollen morphometry characteristics and percentages of pollen viability and in vitro germination. Our results indicate that the highest values of olive pollen viability and germination are associated with highest average values of pollen exine reticulum thickness and diameter.