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Publications

Publications by José Luís Santos

1995

ELECTROOXIDATION OF D-SORBITOL ON PLATINUM IN PERCHLORIC MEDIUM - A VOLTAMMETRIC STUDY

Authors
FONSECA, I; LOPES, MIS; SANTOS, JL; PROENCA, L;

Publication
ELECTROCHIMICA ACTA

Abstract
The electrocatalytic oxidation of D-sorbitol on a polycrystalline platinum electrode, in perchloric aqueous medium, was studied by cyclic voltammetry under varying sweep rate, potential limits, stirring, temperature, pH and concentration of D-sorbitol. A value of about 50 kJ mol(-1) was obtained for the apparent activation enthalpy of the oxidation reaction at 0.1 V vs. sme. The experimental rate equation is i = kC(D-sorb)(0.4) C-H+(-0.3).exp[0.4 FE/RT], valid for [D-sorbitol] < 0.1 mol dm(-3) and pH < 1.

1995

FIBER OPTIC INTERFEROMETRIC SENSOR-BASED ON SOURCE COHERENCE SYNTHESIS BY DYNAMICAL SPECTRAL FILTERING OF A SUPERLUMINESCENT SOURCE

Authors
FERREIRA, LA; SANTOS, JL;

Publication
OPTICS COMMUNICATIONS

Abstract
Interferometric sensors based on source coherence synthesis have been reported for measurement of quasistatic parameters. An absolute sensing technique has been demonstrated based on fringe visibility modulation using a frequency modulated laser. In this Letter, a technique is described which allows to perform absolute displacement measurements in the range from tens to several hundreds of micrometers. It is based on dynamical spectral filtering of a superluminescent source which provides frequency modulation amplitudes up to 3000 GHz. The principle is described and experimentally verified for displacement measurement

1995

POLARIZATION-INSENSITIVE FIBEROPTIC WHITE-LIGHT INTERFEROMETRY

Authors
FERREIRA, LA; SANTOS, JL; FARAHI, F;

Publication
OPTICS COMMUNICATIONS

Abstract
Interferometric systems consisting of two tandem interferometers with white-light illumination have been used as optical-fibre remote-sensing systems. Recently, it has been shown that both phase and amplitude of the output signal are significantly affected by externally induced birefringence in the fibre linking the interferometers; in some cases, this may cause total fading of the interference signal. In this letter, a new scheme is proposed to overcome this problem in white-light fibre-optic interferometric sensors. The approach is based on birefringence compensation using Faraday rotator mirror elements. The principle is experimentally verified, and the results confirm the theoretical expectation.

1998

Demodulation scheme for fiber Bragg grating sensors based on active control of the spectral response of a wavelength division multiplexer

Authors
Araujo, FM; Ferreira, LA; Santos, JL; Farahi, F;

Publication
APPLIED OPTICS

Abstract
We present a closed-loop technique for measuring wavelength shifts associated with fiber Bragg gratings by using a fused biconical wavelength division multiplexer (WDM). The spectral response of the WDM is actively tuned by stretching of the coupling region to maintain a fixed coupling ratio at the reflected Bragg wavelength. The closed-loop operation allows sensitivities usually associated with a highly selective WDM to be obtained without compromising the measurement range. A simple theoretical model is presented together with experimental results for temperature and strain measurements. (C) 1998 Optical Society of America.

2009

Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors

Authors
Santos, JL; Oramas, E; Pego, AP; Granja, PL; Tomas, H;

Publication
JOURNAL OF CONTROLLED RELEASE

Abstract
This paper reports the use of different generations of polyamidoamine (PAMAM) dendrimers for the in vitro transfection of mesenchymal stem cells (MSCs). A systematic study was carried out on the transfection efficiency achieved by the PAMAM dendrimers using a p-galactosidase reporter gene system. Transfection results were shown to be dependent upon the generation of dendrimers, the amine to phosphate group ratio and the cell passage number. In all cases, the transfection efficiency was very low. Nevertheless, it was hypothesized that a low transfection level could be sufficient to promote the in vitro differentiation of MSCs towards the osteoblastic lineage. To address this possibility, dendrimers carrying the human bone morphogenetic protein-2 (hBMP-2) gene-containing plasmid were used. All quantitative (alkaline phosphatase activity, osteocalcin secretion and calcium deposition) and qualitative (von Kossa staining) osteogenic markers were significantly stronger in transfected cells when compared to non-transfected ones. This study not only clearly demonstrates that a low transfection level can be sufficient for inducing in vitro differentiation of MSCs to the osteoblast phenotype but also highlights the importance of focusing research on the development of gene delivery vectors in the concrete application.

2011

Investigation of the long-term stability of arc-induced gratings heat treated at high temperatures

Authors
Rego, G; Caldas, P; Ivanov, O; Santos, JL;

Publication
OPTICS COMMUNICATIONS

Abstract
A long-period grating written in the SMF-28 fibre was heat treated at 1000 degrees C for 15 days. The spectrum of the grating shifted to longer wavelengths and the resonances depth decreased as a result of structural relaxation. The background loss increased considerably for times longer than 200 h, and this loss is caused by devitrification of the fibre.

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