Abstract
Poly(amidoamine) dendrimers (generations 5 and 6) with amine termini were conjugated with peptides containing the arginine-glycine-aspartic acid (RGD) sequence having in view their application as gene delivery vectors. The idea behind the work was to take advantage of the cationic nature of dendrimers and of the integrin targeting capabilities of the RGD motif to improve gene delivery. Dendrimers were used as scaffolds for RGD clustering and, by controlling the number of peptides (4, 8, and 16) linked to each dendrimer, it was possible to evaluate the effect of RGD density on the gene delivery process. The new vectors were characterized in respect to their ability to neutralize and compact plasmid DNA (pDNA). The complexes formed by the vectors and pDNA were studied concerning their size, zeta potential, capacity of being internalized by cells and ability of transferring genes. Transfection efficiency was analyzed, first, by using a pDNA encoding for Enhanced Green Fluorescent Protein and Firefly Luciferase and, second, by using a pDNA encoding for Bone Morphogenetic Protein-2. Gene expression in mesenchymal stem cells was enhanced using the new vectors in comparison to native dendrimers and was shown to be dependent on the electrostatic interaction established between the dendrimer moiety and the cell surface, as well as on the RGD density of nanoclusters. The use of dendrimer scaffolds for RGD cluster formation is a new approach that can be extended beyond gene delivery applications, whenever RGD clustering is important for modulating cellular responses.

Abstract
A reflective multimode interference based-fiber optic sensor is described, aimed at measuring refractive index variations by means of intensity variation based on the fiber tip-interaction concept. The sensing head is a section of a multimode fiber, spliced to a single-mode fiber at the input end; its characteristics are studied when two multimode fibers, with distinct core diameters (viz. 50 and 105 mu m), are considered. The multimode fiber end face is placed in contact with the liquid sample, so as to provide a refractive index measurement via variation in amplitude of the interference spectral peaks - which is essentially insensitive to temperature changes. Therefore, the proposed configuration permits measurement of refractive index variations free from system temperature cross-sensitivity effects. Resolutions of 2.2 x 10(-4) RIU and 3.8 x 10(-4) RIU, for multimode fiber tips with core diameters of 50 mu m and 105 mu m, respectively, could be achieved.

Abstract
An optical fiber sensing system, for monitoring oxygen aiming in vivo nuclear magnetic resonance (NMR) applications is presented. Oxygen detection is based on the dynamic quenching of the fluorescence of a ruthenium complex trapped in the porous structure of a sol-gel silica film. Oxygen concentration is determined by phase-modulation fluorometry. Preliminary results concerning the characterization of doped sol-gel thin films deposited by dip coating in glass slides and in optical fiber probes are presented. Four different probe configurations are tested and compared. Best results are obtained with a fiber taper configuration which shows reproducibility and best excitation efficiency. This structure is fully characterized and some considerations regarding optimal fiber optical sensing probes for 02 detection are addressed.

Abstract
In this work, two all-fiber interferometric configurations based on suspended core fibers (SCF) are investigated. A Fabry-Perot cavity (FPC) made of SCF spliced in-between segments of single-mode and hollow-core fiber is proposed. The interferometric signals are generated by the refractive-index mismatches between the two fibers in the splice region and at the end of the suspended-core fiber. An alternative sensing head configuration formed by the insertion of a length of SCF as a birefringence element in a Sagnac loop interferometer is also demonstrated. In this structure, the interferometric signals are generated by interfering two counter propagating beams with different polarization states which propagate through a length of SCF as a birefringence element. The sensitivity to pressure and temperature was determined for both configurations. The results show that the pressure sensitivities are -4.68 x 10(-5) nm/psi and 0.032 nm/psi for FPC and Sagnac loop interferometers, respectively. The temperature sensitivity of both structures has been obtained and the results have been discussed.

Abstract
A new family of gene delivery vectors is synthesized consisting of a medium-size generation PAMAM dendrimer (generation 5, with amine termini) core randomly linked at the periphery to hydrophobic chains that vary in length (12 to 16 carbon alkyl chains) and number (from 4.2 to 9.7 in average). The idea subjacent to the present work is to join the advantages of the cationic nature of the dendrimer with the capacity of lipids to interact with biological membranes. Unlike other amphiphilic systems designed for the same purpose, where the hydrophobic and hydrophilic moieties coexist in opposite sides, the present vectors have a hydrophilic interior and a hydrophobic corona. The vectors are characterized in respect to their ability to neutralize, bind and compact plasmid DNA (pDNA). The complexes formed between the vectors and pDNA are analyzed concerning their size, zeta-potential, resistance to serum nucleases, capacity of being internalized by cells and transfection efficiency. These new vectors show a remarkable capacity for mediating the internalization of pDNA with minimum cytotoxicity, being this effect positively correlated with the -CH(2)-content present in the hydrophobic corona. Gene expression in MSCs, a cell type with relevancy in the regenerative medicine clinical context, is also enhanced using the new vectors but, in this case, the higher efficiency is shown by the vectors containing the smallest hydrophobic chains.

Abstract
In this study an optical fibre sensor system was conceived for simultaneous detection of the strain and the acoustic emission due to damage. It consists of a fibre Bragg grating and a Fabry-Perot interferometer. For the interrogation of the Fabry-Perot cavity, an innovative low cost process based on the generation of two quadrature phase-shifted signals using two fibre Bragg gratings was implemented, allowing the recovery of the change in the cavity length. An automated processing system, based on Kohonen's self-organizing maps is also presented, for future classification of the acoustic emission waves detected by the optic fibre sensor.