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Publications

Publications by PHT

2021

Particle Classification through the Analysis of the Forward Scattered Signal in Optical Tweezers

Authors
Carvalho, IA; Silva, NA; Rosa, CC; Coelho, LCC; Jorge, PAS;

Publication
SENSORS

Abstract
The ability to select, isolate, and manipulate micron-sized particles or small clusters has made optical tweezers one of the emergent tools for modern biotechnology. In conventional setups, the classification of the trapped specimen is usually achieved through the acquired image, the scattered signal, or additional information such as Raman spectroscopy. In this work, we propose a solution that uses the temporal data signal from the scattering process of the trapping laser, acquired with a quadrant photodetector. Our methodology rests on a pre-processing strategy that combines Fourier transform and principal component analysis to reduce the dimension of the data and perform relevant feature extraction. Testing a wide range of standard machine learning algorithms, it is shown that this methodology allows achieving accuracy performances around 90%, validating the concept of using the temporal dynamics of the scattering signal for the classification task. Achieved with 500 millisecond signals and leveraging on methods of low computational footprint, the results presented pave the way for the deployment of alternative and faster classification methodologies in optical trapping technologies.

2021

Detection of biogenic amines in several foods with different sample treatments: An overview

Authors
Vasconcelos, H; de Almeida, JMMM; Matias, A; Saraiva, C; Jorge, PAS; Coelho, LCC;

Publication
TRENDS IN FOOD SCIENCE & TECHNOLOGY

Abstract
Background: Biogenic amines (BAs) are compounds considered to be contaminants of foodstuff and are cause of poisoning. The main BAs found in foods are cadaverine, putrescine, tyramine, histamine, spermine and spermidine. The number of food poisoning cases associated with BAs in food has increased in the recent years reinforcing the need for early detection to ensure high levels of food quality and safety. Scope and approach: This review aims to provide a general approach to the different BAs detected in foods their concentrations and sample treatments. These compounds are found in varying concentrations in a wide variety of foods such as fish, meat, fruits, vegetables, cheese, wine, and beer. It also refers the different analytical techniques currently used for the detection of BAs, as well as the different treatments of the samples and innovations of the techniques currently used that allow greater sensitivity and speed of the analyzes and with obtaining detection limits lower and lower. Key findings and conclusions: BAs are present in a wide variety of foods and their concentration is highly influenced by the storage conditions of food products. BAs can be precursors of nitrosamines, which have been linked to carcinogenic and mutagenic activity. Several analytical techniques and sample treatments have been improved in the last few years for better and faster detection of BAs.

2021

(INVITED)Classification of optically trapped particles: A comparison between optical fiber tweezers and conventional setups

Authors
Jorge, PAS; Carvalho, IA; Marques, FM; Pinto, V; Santos, PH; Rodrigues, SM; Faria, SP; Paiva, JS; Silva, NA;

Publication
Results in Optics

Abstract
The classification of the type of trapped particles is a crucial task for an efficient integration of optical-tweezers in intelligent microfluidic devices. In the recent years, the use of the temporal scattering signal of the trapped particle paved for the use of versatile optical fiber solutions for performing such tasks, a feature previously unavailable as most methods required conventional optical tweezer setups. This work presents a comprehensive comparison of performances achieved with two distinct implementations – i)optical fiber and ii)conventional optical tweezers – for the classification of the material of particles through the analysis of the scattering signal with machine learning algorithms. The results suggest that while micron-sized particles can be accurately classified using the forward scattering information in conventional optical tweezers, when equipped with a quadrant photodetector, the optical fiber tweezers solutions can easily surpass its performance using the back-scattered information if the laser is modulated. Together with the advantages of being simpler, less expensive and more versatile, the results presented suggest that optical fiber solutions can become a valuable tool for miniaturization and integration of intelligent microfluidic devices working towards nanoscopic scales. © 2021 The Authors

2021

Hydroponics Monitoring through UV-Vis Spectroscopy and Artificial Intelligence: Quantification of Nitrogen, Phosphorous and Potassium

Authors
Silva, AF; Löfkvist, K; Gilbertsson, M; Os, EV; Franken, G; Balendonck, J; Pinho, TM; Boaventura-Cunha, J; Coelho, L; Jorge, P; Martins, RC;

Publication
Chemistry Proceedings

Abstract
In hydroponic cultivation, monitoring and quantification of nutrients is of paramount importance. Precision agriculture has an urgent need for measuring fertilization and plant nutrient uptake. Reliable, robust and accurate sensors for measuring nitrogen (N), phosphorus (P) and potassium (K) are regarded as critical in this process. It is vital to understand nutrients’ interference; thusly, a Hoagland fertilizer solution-based orthogonal experimental design was deployed. Concentration ranges were varied in a target analyte-independent style, as follows: [N] = [103.17–554.85] ppm; [P] = [15.06–515.35] ppm; [K] = [113.78–516.45] ppm, by dilution from individual stock solutions. Quantitative results for N and K, and qualitative results for P were obtained.

2021

Femtosecond laser micromachining of an optofluidics-based monolithic whispering-gallery mode resonator coupled to a suspended waveguide

Authors
Maia, JM; Amorim, VA; Viveiros, D; Marques, PVS;

Publication
SCIENTIFIC REPORTS

Abstract
A monolithic lab-on-a-chip fabricated by femtosecond laser micromachining capable of label-free biosensing is reported. The device is entirely made of fused silica, and consists of a microdisk resonator integrated inside a microfluidic channel. Whispering gallery modes are excited by the evanescent field of a circular suspended waveguide, also incorporated within the channel. Thermal annealing is performed to decrease the surface roughness of the microstructures to a nanometric scale, thereby reducing intrinsic losses and maximizing the Q-factor. Further, thermally-induced morphing is used to position, with submicrometric precision, the suspended waveguide tangent to the microresonator to enhance the spatial overlap between the evanescent field of both optical modes. With this fabrication method and geometry, the alignment between the waveguide and the resonator is robust and guaranteed at all instances. A maximum sensitivity of 121.5 nm/RIU was obtained at a refractive index of 1.363, whereas near the refractive index range of water-based solutions the sensitivity is 40 nm/RIU. A high Q-factor of 10(5) is kept throughout the entire measurement range.

2021

Fiber-Integrated Phase Change Metasurfaces with Switchable Group Delay Dispersion

Authors
Martins, T; Cui, YH; Gholipour, B; Ou, JY; Frazao, O; MacDonald, KF;

Publication
ADVANCED OPTICAL MATERIALS

Abstract
Demonstration of a fiber-integrated non-volatile reconfigurable metasurface providing high-contrast group delay dispersion switching functionality is reported, which may be engineered to operate at wavelengths across the near-infrared (telecoms) band. Light-induced amorphous-crystalline phase switching in a chalcogenide (germanium antimony telluride) metasurface, only a fraction of a wavelength thick, fabricated on the end-facet of a single-mode optical fiber, enables intensity and phase modulation of the guided wave at metasurface designated bands. Such devices present a range of opportunities in fiberized remotely programmable phase/intensity multiplexing and dynamic dispersion compensation for emerging telecommunications and data storage/processing applications, including in photonic neural network and neuromorphic computing architectures.

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