Abstract
A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62 +/- 2 nM and the limit of quantification was 209 +/- 7 nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.

Abstract
Core diameter mismatch structures are proposed and experimentally investigated for curvature and vibration sensing. Two configurations are suggested, one approach uses a structure formed by splicing an uncoated short section of multimode fiber between two standard single-mode fibers (SMFs) single-mode-multimode-single-mode (SMS), combined to a fiber optical mirror at its end, and the other approach uses a structure made by splicing a section of SMF between two multimode fibers (SMSMS). In the curvature analysis, the proposed SMS sensor generates the destructive interference patterns when it is bent, varying only the attenuation of the optical signal without wavelength shifts. The SMSMS vibration sensor proved to be suitable to monitor very low frequencies such as 0.1 Hz. The configuration of the proposed sensors presents several interesting features, such as easy fabrication, low cost, high efficiency, and high sensitivity. These advantages make such sensors very useful in a wide range of applications, for instance, structural health monitoring.

Abstract
This work presents an autonomous system for marine integrated physical-chemical and biological monitoring - the MarinEye system. It comprises a set of sensors providing diverse and relevant information for oceanic environment characterization and marine biology studies. It is constituted by a physical-chemical water properties sensor suite, a water filtration and sampling system for DNA collection, a plankton imaging system and biomass assessment acoustic system. The MarinEye system has onboard computational and logging capabilities allowing it either for autonomous operation or for integration in other marine observing systems (such as Observatories or robotic vehicles. It was designed in order to collect integrated multi-trophic monitoring data. The validation in operational environment on 3 marine observatories: RAIA, BerlengasWatch and Cascais on the coast of Portugal is also discussed.

Abstract
This article addresses the design, development, and evaluation of T-shirt prototypes that embed novel textile sensors for the capture of cardio and respiratory signals. The sensors are connected through textile interconnects to either an embedded custom-designed data acquisition and transmission unit or to snap fastener terminals for connection to external monitoring devices. The performance of the T-shirt prototype is evaluated in terms of signal-to-noise ratio amplitude and signal interference caused by baseline wander and motion artefacts, through laboratory tests with subjects in standing and walking conditions. Performance tests were also conducted in a hospital environment using a T-shirt prototype connected to a commercial three-channel Holter monitoring device. The textile sensors and interconnects were realized with the assistance of an industrial six-needle digital embroidery tool and their resistance to wear addressed with normalized tests of laundering and abrasion. The performance of these wearable systems is discussed, and pathways and methods for their optimization are highlighted.

Abstract
This paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead counting.

Abstract
In this work, spiral phase lenses fabricated on the tip of single mode optical fibers are reported. This allows tailoring the fundamental guided mode, a Gaussian beam, into a Laguerre - Gaussian profile without using additional optical elements. The lenses are fabricated using Focused Ion Beam milling, enabling high resolution in the manufacturing process. The phase profiles are evaluated and validated using an implementation of the Finite Differences Time Domain. The output optical intensity profiles matching the numerical simulations are presented and analyzed. Finally, results on cell trapping and manipulation are briefly described.