Abstract
Sensing at the single cell level can provide insights into its dynamics and heterogeneity, yielding information otherwise unattainable with traditional biological methods where average population behavior is observed. In this context, optical tweezers provide the ability to select, separate, manipulate and identify single cells or other types of microparticles, potentially enabling single cell diagnostics. Forward or backscatter analysis of the light interacting with the trapped cells can provide valuable insights on the cell optical, geometrical and mechanical properties. In particular, the combination of tweezers systems with advanced machine learning algorithms can enable single cell identification capabilities. However, typical processing pipelines require a training stage which often struggles when trying to generalize to new sets of data. In this context, fully automated tweezers system can provide mechanisms to obtain much larger datasets with minimum effort form the users, while eliminating procedural variability. In this work, a pipeline for full automation of optical tweezers systems is discussed. A performance comparison between manually operated and fully automated tweezers systems is presented, clearly showing advantages of the latter. A case study demonstrating the ability of the system to discriminate molecular binding events on microparticles is presented.

Abstract
This study estimates alighting stops and transfers from entry-only Automatic Fare Collection (AFC) data. The methodology adopted includes two main steps: an implementation of the Trip Chaining Method (TCM) to estimate the alighting stops from AFC records and the subsequent application of criteria for the identification of transfers. For each pair of consecutive AFC records on the same smart card, a transfer is identified considering a threshold for the walking distance, a threshold for the time required to perform an activity, and the validation of different boarding routes. This methodology was applied to the case study of Porto, Portugal, considering all trips performed by a set of 19999 smart cards over one year. The results of this methodology allied with visualization techniques allowed to study Origin-Destination (OD) patterns by type of day, seasonally, and by user frequency, each analyzed at the stop level and at the geographic area level.

Abstract
Recent advancements in digital technology have significantly impacted healthcare, with the rise of chatbots as a promising avenue for healthcare services. These chatbots aim to provide prevention, diagnosis, and treatment services, thereby reducing the workload on medical professionals. Despite this trend, limited research has explored the variables influencing user experiences in the design of healthcare chatbots. While the impact of visual representation within chatbot systems is recognized, existing studies have primarily focused on efficiency and accuracy, neglecting graphical interfaces and non-verbal visual communication tools. This research aims to delve into user experience aspects of symptom checker chatbots, including identity design, interface layout, and visual communication mechanisms. Data was collected through a comprehensive questionnaire involving three distinct chatbots (Healthily, Mediktor and Adele - a self-developed solution) and underwent meticulous analysis, yielding valuable insights to aid the decision process when designing effective chatbots for symptom checking.

Abstract
The technological revolution experienced over the last two decades, together with changes in shopping behaviour, has led supermarkets to consider smart shopping trolleys. Recently, several companies have tested and implemented smart services and devices, such as smart shopping carts with scanners, automatic payment methods, or self-payment locations, to maximise supermarket profits by reducing staff and improving the customer experience. In the spring of 2023, a team of six students enrolled in the European Project Semester at Instituto Superior de Engenharia do Porto (ISEP) proposed FESmarket, an innovative smart shopping cart solution. The user-centred design focused on making the shopping interaction and experience more efficient, comfortable, and satisfactory. Form (balancing aesthetics with innovation), function (selecting functionalities based on the most disruptive technologies), market (fulfilling the identified needs), sustainability (minimising the use of resources), and ethics (respecting human values) are the pillars of the project. FESmarket proposes a smart shopping trolley equipped a built-in touch screen for real-time information on products and their location, cameras for product identification, an audio assistance system, a refrigeration chamber, and a mobile app interface for the customer. Finally, a proof-of-concept prototype was assembled and tested to validate the viability of the designed solution. © The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2024.

Abstract
Recently, multi-carrier energy systems (MCESs) have been rapidly developed as flexible multi-generation systems aiming to satisfy load demands by purchasing, converting, and storing different energy carriers. This study specifically focuses on the optimal and robust large-scale coordination of interconnected energy hubs (IEHs) in an iterative consensus-based procedure considering distribution network losses. Furthermore, a new robustbased hybrid IGDT/consensus algorithm is introduced to achieve risk-averse optimal energy management in IEHs under uncertainty. The fast convergence, needless to collect the total information from all hubs, minimal computational burden, and more robust communication system are the most important features of the proposed distributed consensus algorithm in this study. The effectiveness of the proposed consensus algorithm is verified by simulation results considering various energy trading structures in IEHs at different scales. The obtained results highlight the scalability capability of the proposed method. Regarding an IEHS of 30 energy hubs, the computation burden is lightened by 0.53 (s) and 0.1917 (s), respectively with and without uncertainty. Considering distribution network losses, the total purchasing costs can be increased by 8%. The simulation results also reveal an increase of 11% in the total power trading under the uncertainty.

Abstract
In the health sector, the implementation of qualitative data research is very important to improve overall services. However, the use of these methods remains relatively unexplored when compared to quantitative analyses. This article describes the qualitative data analysis process that is based on the description, analysis and interpretation of data. It also describes a practical case study and the use of NVivo software to assist in the development of a theory-based qualitative analysis process. This article intends to be a step forward in the use of qualitatively based methodologies in future research in the health sector.