2026
Authors
Toribio, L; Veloso, B; Gama, J; Zafra, A;
Publication
NEUROCOMPUTING
Abstract
Early fault detection remains a critical challenge in predictive maintenance (PdM), particularly within critical infrastructure, where undetected failures or delayed interventions can compromise safety and disrupt operations. Traditional anomaly detection methods are typically reactive, relying on real-time sensor data to identify deviations as they occur. This reactive nature often provides insufficient lead time for effective maintenance planning. To address this limitation, we propose a novel two-stage early detection framework that integrates time series forecasting with anomaly detection to anticipate equipment failures several hours in advance. In the first stage, future sensor signal values are predicted using forecasting models; in the second, conventional anomaly detection algorithms are applied directly to the forecasted data. By shifting from real-time to anticipatory detection, the framework aims to deliver actionable early warnings, enabling timely and preventive maintenance. We validate this approach through a case study focused on metro train systems, an environment where early fault detection is crucial for minimizing service disruptions, optimizing maintenance schedules, and ensuring passenger safety. The framework is evaluated across three forecast horizons (1, 3, and 6 hours ahead) using twelve state-of-the-art anomaly detection algorithms from diverse methodological families. Detection performance is assessed using five performance metrics. Results show that anomaly detection remains highly effective at short to medium horizons, with performance at 1-hour and 3-hour forecasts comparable to that of real-time data. Ensemble and deep learning models exhibit strong robustness to forecast uncertainty, maintaining consistent results with real-time data even at 6-hour forecasts. In contrast, distance- and density-based models suffer substantial degradation at longer horizons (6-hours), reflecting their sensitivity to distributional shifts in predicted signals. Overall, the proposed framework offers a practical and extensible solution for enhancing traditional PdM systems with proactive capabilities. By enabling early anomaly detection on forecasted data, it supports improved decision-making, operational resilience, and maintenance planning in industrial environments.
2026
Authors
Garcia, A; Martinez, M; Marco, TS; Almeida, FL;
Publication
Business Sustainability: Innovation in Entrepreneurship & Internationalisation
Abstract
2026
Authors
Cammaerts, F; Tramontana, P; Flores, N; Doorn, N; Fasolino, AR; Marin, B; Paiva, ACR; Vos, TEJ; Snoeck, M;
Publication
Abstract
2026
Authors
Khan, SN; Iqbal, A; Almeida, FL;
Publication
Business Sustainability: Innovation in Entrepreneurship & Internationalisation
Abstract
2026
Authors
Teixeira, J; Ribeiro, JA; Monteiro, M; Silva, NA; Jorge, PAS;
Publication
SENSORS
Abstract
The ability to assess molecular binding kinetics in real time is critical for advancing our understanding of molecular interactions in biochemical and biotechnological systems. This work presents a novel optical tweezer (OT)-based method to monitor molecular affinity in real time, focusing on the high-affinity streptavidin-biotin system as a model. Transparent poly(methyl methacrylate) (PMMA) microparticles functionalized with streptavidin were trapped before, during, and after binding with biotinylated bovine serum albumin (biotin-BSA), enabling the analysis of forward-scattered signals to detect nanoscale changes in particle size. By applying the Power Spectral Density method, the friction coefficient of individual particles was calculated, allowing for real-time tracking of binding dynamics and the estimation of the association rate constant (kon approximate to 106M-1s-1). These results are consistent with literature values and demonstrate the potential of this OT-based approach for non-invasive, label-free detection of molecular interactions. Compared to existing techniques, such as atomic force microscopy and cantilever-based sensors, this method offers significant advantages, including real-time monitoring, adaptability to different bioaffinity systems, and compatibility with miniaturized setups. This work establishes a foundation for using OT-based tools to monitor high-affinity molecular interactions in real time. While demonstrated here using biotinylated BSA as a model ligand, future studies will explore the method's applicability to smaller ligands and more subtle surface modifications.
2026
Authors
Saadatmand, M; Khan, A; Marin, B; Paiva, ACR; Van Asch, N; Moran, G; Cammaerts, F; Snoeck, M; Mendes, A;
Publication
PRODUCT-FOCUSED SOFTWARE PROCESS IMPROVEMENT. INDUSTRY, DOCTORAL-SYMPOSIUM, TUTORIAL, AND WORKSHOP PAPERS, PROFES 2025
Abstract
The evolving landscape of software development demands that software testers continuously adapt to new tools, practices, and acquire new skills. This study investigates software testing competency needs in industry, identifies knowledge gaps in current testing education, and highlights competencies and gaps not addressed in academic literature. This is done by conducting two focus group sessions and interviews with professionals across diverse domains, including railway industry, healthcare, and software consulting and performing a curated small-scale scoping review. The study instrument, co-designed by members of the ENACTEST project consortium, was developed collaboratively and refined through multiple iterations to ensure comprehensive coverage of industry needs and educational gaps. In particular, by performing a thematic qualitative analysis, we report our findings and observations regarding: professional training methods, challenges in offering training in industry, different ways of evaluating the quality of training, identified knowledge gaps with respect to academic education and industry needs, future needs and trends in testing education, and knowledge transfer methods within companies. Finally, the scoping review results confirm knowledge gaps in areas such as AI testing, security testing and soft skills.
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