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Publications

Publications by Luís Carlos Coelho

2021

Label-Free Anti-Human IgG Biosensor Based on Chemical Modification of a Long Period Fiber Grating Surface

Authors
Mendes, JP; Coelho, LCC; Pereira, VP; Azenha, MA; Jorge, PAS; Pereira, CM;

Publication
Chemistry Proceedings

Abstract
This work introduces a method specially developed to produce a biorecognition element based on modified Stöber silica nanoparticles by the covalent immobilization of the human IgG. The sensing structure is based on long period fiber gratings (LPFG), specially developed to allow the interaction of the electromagnetic wave with the target analytes through its evanescent field. The surface was modified by the immobilization of the IgG-modified nanoparticles serving has recognition elements for specific target molecules. The resulting configuration was tested in the presence of anti-human IgG, recording the refractometric response of the modified LPFG in contact with different amounts of analyte. The selectivity of the sensor was also assessed.

2021

Optical Biosensor for the Detection of Hydrogen Peroxide in Milk

Authors
Vasconcelos, H; Matias, A; Jorge, P; Saraiva, C; Mendes, J; Araújo, J; Dias, B; Santos, P; Almeida, JMMM; Coelho, LCC;

Publication
Chemistry Proceedings

Abstract
Over the years, the food industry’s concern to provide safe food that does not cause harm or illness to consumers has increased. The growing demand for the detection of compounds that can contaminate food is increasingly important. Hydrogen peroxide is frequently used as a substance to control the growth of microorganisms in milk, thus increasing its shelf life. Here, a strategy is presented for the detection of hydrogen peroxide as a milk adulterant, using a single shot membrane sensor. The lowest concentration measured with this technique was 0.002% w/w of H2O2 in semi-fat milk.

2021

Single Fiber Reflectance Spectroscopy for the Monitoring of Cement Paste

Authors
da Silva, PM; Coelho, LCC; Almeida, JMMMD;

Publication
CHEMOSENSORS

Abstract
Reinforced concrete structures are an essential part of our modern society, and monitoring their structural health is of paramount importance. Early detection of decay allows for the reduction of repair costs and, more importantly, the prevention of catastrophic failure. For this purpose, a single fiber reflectance spectrometer was embedded in cement paste samples for the monitoring of water at the fiber tip through its sensitivity to changes in the refractive index. It monitored the curing of samples with different water-to-cement ratios (w/c), between 0.45 and 0.60, measuring the water exhaust during the hardening of the cement paste. It also measured the capillary coefficient from cement paste samples of 0.50, 0.55 and 0.60 w/c: 0.668 & PLUSMN; 0.002 mm/& RADIC;h, 1.771 & PLUSMN; 0.052 mm/& RADIC;h and 6.360 & PLUSMN; 0.269 mm/& RADIC;h, respectively. The capillary coefficient values agree with gravimetric measurements of sorptivity and are further confirmed through porosity measurements made with a scanning electron microscope. Thus, single fiber reflectance spectroscopy can be a gateway to inexpensively measure the entire life cycle of cement, from its curing until its eventual decay, assessing, in situ, its durability through the capillary coefficient.

2021

Characterization and Comparison of the Relative Humidity Response of Hydromorphic Polymers in Long-Period Fiber Grating Structures

Authors
Dias, B; Mendes, JP; de Almeida, JMMM; Coelho, LCC;

Publication
Chemistry Proceedings

Abstract
Relative humidity monitorization is of extreme importance on scientific and industrial applications, and fiber optics-based sensors may provide solutions where other types of sensors have limitations. In this work, fiber optics’ sensors were fabricated by combining Long-Period Fiber Gratings with three different humidity-responding polymers, namely Poly(vinyl alcohol), Poly(ethylene glycol) and Hydromed™ D4. The performance of the multiple sensors was experimentally tested and crossed with numerical simulations, which provide a comparison with the expected response given the optical properties of the materials.

2022

Analysis of the Relative Humidity Response of Hydrophilic Polymers for Optical Fiber Sensing

Authors
Dias, B; Carvalho, J; Mendes, JP; Almeida, JMMM; Coelho, LCC;

Publication
POLYMERS

Abstract
Relative humidity (RH) monitorization is of extreme importance on scientific and industrial applications, and optical fiber sensors (OFS) may provide adequate solutions. Typically, these kinds of sensors depend on the usage of humidity responsive polymers, thus creating the need for the characterization of the optical and expansion properties of these materials. Four different polymers, namely poly(vinyl alcohol), poly(ethylene glycol), Hydromed (TM) D4 and microbiology agar were characterized and tested using two types of optical sensors. First, optical fiber Fabry-Perot (FP) tips were made, which allow the dynamical measurement of the polymers' response to RH variations, in particular of refractive index, film thickness, and critical deliquescence RH. Using both FP tips and Long-Period fiber gratings, the polymers were then tested as RH sensors, allowing a comparison between the different polymers and the different OFS. For the case of the FP sensors, the PEG tips displayed excellent sensitivity above 80%RH, outperforming the other polymers. In the case of LPFGs, the 10% (wt/wt) PVA one displayed excellent sensitivity in a larger working range (60 to 100%RH), showing a valid alternative to lower RH environment sensing.

2021

Hydroponics Monitoring through UV-Vis Spectroscopy and Artificial Intelligence: Quantification of Nitrogen, Phosphorous and Potassium

Authors
Silva, AF; Löfkvist, K; Gilbertsson, M; Os, EV; Franken, G; Balendonck, J; Pinho, TM; Boaventura-Cunha, J; Coelho, L; Jorge, P; Martins, RC;

Publication
Chemistry Proceedings

Abstract
In hydroponic cultivation, monitoring and quantification of nutrients is of paramount importance. Precision agriculture has an urgent need for measuring fertilization and plant nutrient uptake. Reliable, robust and accurate sensors for measuring nitrogen (N), phosphorus (P) and potassium (K) are regarded as critical in this process. It is vital to understand nutrients’ interference; thusly, a Hoagland fertilizer solution-based orthogonal experimental design was deployed. Concentration ranges were varied in a target analyte-independent style, as follows: [N] = [103.17–554.85] ppm; [P] = [15.06–515.35] ppm; [K] = [113.78–516.45] ppm, by dilution from individual stock solutions. Quantitative results for N and K, and qualitative results for P were obtained.

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