Abstract
Microsatellite Instability (MSI) is an important biomarker in cancer patients, showing a defective DNA mismatch repair system. Its detection allows the use of immunotherapy to treat cancer, an approach that is revolutionizing cancer treatment. MSI is especially relevant for three types of cancer: Colon Adenocarcinoma (COAD), Stomach Adenocarcinoma (STAD), and Uterus corpus endometrial cancer (UCEC). In this work, learning algorithms were employed to predict MSI using RNA-seq data from The Cancer Genome Atlas (TCGA) database, with a focus on the selection of the most informative genomic features. The Multi-Layer Perceptron (MLP) obtained the best score (AUC = 98.44%), showing that it is possible to exploit information from RNA-seq data to find relevant relationships with the instability levels of microsatellites (MS). The accurate prediction of MSI with transcription data from cancer patients will help with the correct determination of MSI status and adequate prescription of immunotherapy, creating more precise and personalized patient care. At the genetic level, the study revealed a high expression of genes related to cell regulation functions, and a low expression of genes responsible for Mismatch Repair functions, in patients with high instability.

Abstract
With the objective of developing new methods to acquire diagnostic information, the reconstruction of the broadband absorption coefficient spectra (mu a[lambda]) of healthy and chromophobe renal cell carcinoma kidney tissues was performed. By performing a weighted sum of the absorption spectra of proteins, DNA, oxygenated, and deoxygenated hemoglobin, lipids, water, melanin, and lipofuscin, it was possible to obtain a good match of the experimental mu a(lambda) of both kidney conditions. The weights used in those reconstructions were estimated using the least squares method, and assuming a total water content of 77% in both kidney tissues, it was possible to calculate the concentrations of the other tissue components. It has been shown that with the development of cancer, the concentrations of proteins, DNA, oxygenated hemoglobin, lipids, and lipofuscin increase, and the concentration of melanin decreases. Future studies based on minimally invasive spectral measurements will allow cancer diagnosis using the proposed approach.

Abstract
Climate change is causing the emergence of new pest species and diseases, threatening economies, public health, and food security. In Europe, olive groves are crucial for producing olive oil and table olives; however, the presence of the olive fruit fly (Bactrocera Oleae) poses a significant threat, causing crop losses and financial hardship. Early disease and pest detection methods are crucial for addressing this issue. This work presents a pioneering comparative performance study between two state-of-the-art object detection models, YOLOv5 and YOLOv8, for the detection of the olive fruit fly from trap images, marking the first-ever application of these models in this context. The dataset was obtained by merging two existing datasets: the DIRT dataset, collected in Greece, and the CIMO-IPB dataset, collected in Portugal. To increase its diversity and size, the dataset was augmented, and then both models were fine-tuned. A set of metrics were calculated, to assess both models performance. Early detection techniques like these can be incorporated in electronic traps, to effectively safeguard crops from the adverse impacts caused by climate change, ultimately ensuring food security and sustainable agriculture. © 2024 by SCITEPRESS – Science and Technology Publications, Lda.

Abstract

Abstract
Significant medical image visualization and annotation tools, tailored for clinical users, play a crucial role in disease diagnosis and treatment. Developing algorithms for annotation assistance, particularly machine learning (ML)-based ones, can be intricate, emphasizing the need for a user-friendly graphical interface for developers. Many software tools are available to meet these requirements, but there is still room for improvement, making the research for new tools highly compelling. The envisioned tool focuses on navigating sequences of DICOM images from diverse modalities, including Magnetic Resonance Imaging (MRI), Computed Tomography (CT) scans, Ultrasound (US), and X-rays. Specific requirements involve implementing manual annotation features such as freehand drawing, copying, pasting, and modifying annotations. A scripting plugin interface is essential for running Artificial Intelligence (AI)-based models and adjusting results. Additionally, adaptable surveys complement graphical annotations with textual notes, enhancing information provision. The user evaluation results pinpointed areas for improvement, including incorporating some useful functionalities, as well as enhancements to the user interface for a more intuitive and convenient experience. Despite these suggestions, participants praised the application's simplicity and consistency, highlighting its suitability for the proposed tasks. The ability to revisit annotations ensures flexibility and ease of use in this context.

Abstract
The early and accurate detection and the grading characterization of brain cancer will generate a positive impact on the treatment plan of those patients. AI-based models can help analyze the Magnetic Resonance Imaging (MRI) to make an initial assessment of the tumor grading. The objective of this work was to develop an Al-based model to classify the grading of the tumor using the MRI. Two regions of interest were explored, with several levels of complexity for the neural network architecture, and Iwo strategies to deal with Unbalanced data. The best results were obtained for the most complex architecture (Resnet50) with a combination of weighted random sampler and data augmentation achieving a balanced accuracy of 62.26%. This work confirmed that complex problems required a more dense neural network and strategies to deal with the unbalanced data.