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Publicações

Publicações por CAP

2022

Towards real-time identification of trapped particles with UMAP-based classifiers

Autores
Teixeira, J; Rocha, V; Oliveira, J; Jorge, PAS; Silva, NA;

Publicação
Journal of Physics: Conference Series

Abstract
Optical trapping provides a way to isolate, manipulate, and probe a wide range of microscopic particles. Moreover, as particle dynamics are strongly affected by their shape and composition, optical tweezers can also be used to identify and classify particles, paving the way for multiple applications such as intelligent microfluidic devices for personalized medicine purposes, or integrated sensing for bioengineering. In this work, we explore the possibility of using properties of the forward scattered radiation of the optical trapping beam to analyze properties of the trapped specimen and deploy an autonomous classification algorithm. For this purpose, we process the signal in the Fourier domain and apply a dimensionality reduction technique using UMAP algorithms, before using the reduced number of features to feed standard machine learning algorithms such as K-nearest neighbors or random forests. Using a stratified 5-fold cross-validation procedure, our results show that the implemented classification strategy allows the identification of particle material with accuracies up to 80%, demonstrating the potential of using signal processing techniques to probe properties of optical trapped particles based on the forward scattered light. Furthermore, preliminary results of an autonomous implementation in a standard experimental optical tweezers setup show similar differentiation capabilities for real-time applications, thus opening some opportunities towards technological applications such as intelligent microfluidic devices and solutions for biochemical and biophysical sensing. © Published under licence by IOP Publishing Ltd.

2022

Automation strategies and machine learning algorithms towards real-time identification of optically trapped particles

Autores
Oliveira, J; Rocha, V; Silva, NA; Jorge, PAS;

Publicação
EPJ Web of Conferences

Abstract
To automatically trap, manipulate and probe physical properties of micron-sized particles is a step of paramount importance for the development of intelligent and integrated optomicrofluidic devices. In this work, we aim at implementing an automatic classifier of micro-particles immersed in a fluid based on the concept of optical tweezers. We describe the automation steps of an experimental setup together with the implemented classification models using the forward scattered signal. The results show satisfactory accuracy around 80% for the identification of the type and size of particles using signals of 250 milliseconds of duration, which paves the path for future improvements towards real-time analysis of the trapped specimens.

2022

Autonomous Optical Tweezers: From automatic trapping to single particle analysis

Autores
Coutinho, F; Teixeira, J; Rocha, V; Oliveira, J; Jorge, PAS; Silva, NA;

Publicação
Journal of Physics: Conference Series

Abstract
Optical trapping is a versatile and non-invasive technique for single particle manipulation. As such, it can be widely applied in the domains of particle identification and classification and thus used as a tool for monitoring physical and chemical processes. This creates an opportunity for integrating the method seamlessly into optofluidic chips, provided it can be automatized. Yet even though OT is well established in multiple scientific domains, a full stack approach to its integration into other technological devices is still lacking. This calls for solutions in tasks such as automatic trapping and signal analysis. In this manuscript, we describe the implementation of an algorithm seeking autonomous particle location and trapping. The methodology is based upon image-processing, allowing for particle location using real time image segmentation. A local thresholding algorithm is applied, followed by morphological techniques for closing shapes and excluding non-bounded regions - after which only the particles remain on the image. Once the centroid is identified, the stage is translated accordingly by piezo-electric actuators, followed by the laser activation. In this way, trapping is achieved, and one may proceed to analyze the forward scattered optical signal, after which a new particle inside the actuators range may be automatically trapped. This development, when compared with existent solutions involving holographic optical tweezers, allows for similar capabilities without using a spatial light modulator, thus dramatically reducing the setup costs of autonomous OT solutions. Therefore, when combined with particle classification techniques, this method is well suited for integration into possible optofluidic chips for autonomous sensing and monitoring of biochemical samples. © Published under licence by IOP Publishing Ltd.

2022

Differential Refractometric Platform for Reliable Biosensing based on Long-period Gratings and Molecular Imprinting

Autores
Mendes, JP; Coelho, LCC; Pereira, CM; Jorge, PAS;

Publicação
Optics InfoBase Conference Papers

Abstract
A new (bio)sensing platform based on differential refractometric measurements was developed. The sensing scheme is based on the combination LPFGs/MIP/NIP, involving a dual channel system for real-time compensation of non-specific interactions. The correction system improves the sensor behavior by reducing the response to interferents by 30%. © 2022 The Author(s).

2022

High Sensitivity Cryogenic Temperature Sensors Based on Arc-Induced Long-Period Fiber Gratings

Autores
Ivanov, OV; Caldas, P; Rego, G;

Publicação
SENSORS

Abstract
In this paper, we investigated the evolution of the dispersion curves of long-period fiber gratings (LPFGs) from room temperature down to 0 K. We considered gratings arc-induced in the SMF28 fiber and in two B/Ge co-doped fibers. Computer simulations were performed based on previously published experimental data. We found that the dispersion curves belonging to the lowest-order cladding modes are the most affected by the temperature changes, but those changes are minute when considering cladding modes with dispersion turning points (DTP) in the telecommunication windows. The temperature sensitivity is higher for gratings inscribed in the B/Ge co-doped fibers near DTP and the optimum grating period can be chosen at room temperature. A temperature sensitivity as high as -850 pm/K can be obtained in the 100-200 K temperature range, while a value of -170 pm/K is reachable at 20 K.

2022

A ?-Shaped Bending-Optical Fiber Sensor for the Measurement of Radial variation in Cylindrical Structures

Autores
Cardoso, V; Caldas, P; Giraldi, MTR; Frazão, O; Costa, J; Santos, JL;

Publicação
EPJ Web of Conferences

Abstract
This work presents preliminary results of the ? -shaped sensor mounted on support designed by additive manufacturing (AM). This sensor is proposed and experimentally demonstrated to measure the radial variation of cylindrical structures. The sensor presents an easy fabrication. The support was developed to work using the principle of leverage. The sensing head is curled between two points so that the dimension associated with the macro bend is changed when there is a radial variation. The results indicate that the proposed sensor structure can monitor radial variation in applications such as pipelines and trees.

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