Abstract
Sound-based uroflowmetry (SU) is a non-invasive technique emerging as an alternative to traditional uroflowmetry (UF) to calculate the voiding flow rate based on the sound generated by the urine impacting the water in a toilet, enabling remote monitoring and reducing the patient burden and clinical costs. This study trains four different machine learning (ML) models (random forest, gradient boosting, support vector machine and convolutional neural network) using both regression and classification approaches to predict and categorize the voiding flow rate from sound events. The models were trained with a dataset that contains sounds from synthetic void events generated with a high precision peristaltic pump and a traditional toilet. Sound was simultaneously recorded with three devices: Ultramic384k, Mi A1 smartphone and Oppo Smartwatch. To extract the audio features, our analysis showed that segmenting the audio signals into 1000 ms segments with frequencies up to 16 kHz provided the best results. Results show that random forest achieved the best performance in both regression and classification tasks, with a mean absolute error (MAE) of 0.9, 0.7 and 0.9 ml/s and quadratic weighted kappa (QWK) of 0.99, 1.0 and 1.0 for the three devices. To evaluate the models in a real environment and assess the effectiveness of training with synthetic data, the best-performing models were retrained and validated using a real voiding sounds dataset. The results reported an MAE below 2.5 ml/s and a QWK above 0.86 for regression and classification tasks, respectively.

Abstract
BackgroundTobacco smoke is the main cause of preventable mortality worldwide. Smoking increases the risk of developing many diseases and has been proposed as an aging accelerator. Yet, the molecular mechanisms driving smoking-related health decline and aging acceleration in most tissues remain unexplored.MethodsHere, we use data from the Genotype-Tissue Expression Project (GTEx) to perform a characterization of the effect of cigarette smoking across human tissues. We perform a multi-tissue analysis across 46 human tissues. Our multi-omics characterization includes analysis of gene expression, alternative splicing, DNA methylation, and histological alterations. We further analyze ex-smoker samples to assess the reversibility of these molecular alterations upon smoking cessation.ResultsWe show that smoking impacts tissue architecture and triggers systemic inflammation. We find that in many tissues, the effects of smoking significantly overlap those of aging. Specifically, both age and smoking upregulate inflammatory genes and drive hypomethylation at enhancers (odds ratio (OR) = 2). In addition, we observe widespread smoking-driven hypermethylation at target regions of the Polycomb repressive complex (OR = 2), which is a well-known aging effect. Smoking-induced epigenetic changes overlap causal aging CpGs, suggesting that these methylation changes may directly mediate the aging acceleration observed in smokers. Finally, we find that smoking effects that are shared with aging are more persistent over time.ConclusionOverall, our multi-tissue and multi-omic analysis of the effects of cigarette smoking provides an extensive characterization of the impact of tobacco smoke across tissues and unravels the molecular mechanisms driving smoking-induced tissue homeostasis decline and aging acceleration.

Abstract
Breakthroughs in sequencing technologies led to an exponential growth of genomic data, providing novel biological insights and therapeutic applications. However, analyzing large amounts of sensitive data raises key data privacy concerns, specifically when the information is outsourced to untrusted third-party infrastructures for data storage and processing (e.g., cloud computing). We introduce Gyosa, a secure and privacy-preserving distributed genomic analysis solution. By leveraging trusted execution environments (TEEs), Gyosa allows users to confidentially delegate their GWAS analysis to untrusted infrastructures. Gyosa implements a computation partitioning scheme that reduces the computation done inside the TEEs while safeguarding the users' genomic data privacy. By integrating this security scheme in Glow, Gyosa provides a secure and distributed environment that facilitates diverse GWAS studies. The experimental evaluation validates the applicability and scalability of Gyosa, reinforcing its ability to provide enhanced security guarantees.

Abstract

Abstract
Multivariate time series analysis is a vital but challenging task, with multidisciplinary applicability, tackling the characterization of multiple interconnected variables over time and their dependencies. Traditional methodologies often adapt univariate approaches or rely on assumptions specific to certain domains or problems, presenting limitations. A recent promising alternative is to map multivariate time series into high-level network structures such as multiplex networks, with past work relying on connecting successive time series components with interconnections between contemporary timestamps. In this work, we first define a novel cross-horizontal visibility mapping between lagged timestamps of different time series and then introduce the concept of multilayer horizontal visibility graphs. This allows describing cross-dimension dependencies via inter-layer edges, leveraging the entire structure of multilayer networks. To this end, a novel parameter-free topological measure is proposed and common measures are extended for the multilayer setting. Our approach is general and applicable to any kind of multivariate time series data. We provide an extensive experimental evaluation with both synthetic and real-world datasets. We first explore the proposed methodology and the data properties highlighted by each measure, showing that inter-layer edges based on cross-horizontal visibility preserve more information than previous mappings, while also complementing the information captured by commonly used intra-layer edges. We then illustrate the applicability and validity of our approach in multivariate time series mining tasks, showcasing its potential for enhanced data analysis and insights.

Abstract
The presence of missing data poses a common challenge for time series analysis in general since the most usual requirement is that the data is equally spaced in time and therefore imputation methods are required. For time series of counts, the usual imputation methods which usually produce real valued observations, are not adequate. This work employs Bayesian principles for handling missing data within time series of counts, based on first-order integer-valued autoregressive (INAR) models, namely Approximate Bayesian Computation (ABC) and Gibbs sampler with Data Augmentation (GDA) algorithms. The methodologies are illustrated with synthetic and real data and the results indicate that the estimates are consistent and present less bias when the percentage of missing observations decreases, as expected. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2025.