Abstract
Eukaryotic cells make many types of primary and processed RNAs that are found either in specific subcellular compartments or throughout the cells. A complete catalogue of these RNAs is not yet available and their characteristic subcellular localizations are also poorly understood. Because RNA represents the direct output of the genetic information encoded by genomes and a significant proportion of a cell's regulatory capabilities are focused on its synthesis, processing, transport, modification and translation, the generation of such a catalogue is crucial for understanding genome function. Here we report evidence that three-quarters of the human genome is capable of being transcribed, as well as observations about the range and levels of expression, localization, processing fates, regulatory regions and modifications of almost all currently annotated and thousands of previously unannotated RNAs. These observations, taken together, prompt a redefinition of the concept of a gene.

Abstract
Alternative splicing is the mechanism by which different combinations of exons in the pre-mRNA give rise to distinct mature mRNAs. This process is mediated by splicing factors that bind the pre-mRNA and affect the recognition of its splicing signals. Saccharomyces species lack many of the regulatory factors present in metazoans. Accordingly, it is generally assumed that the amount of alternative splicing is limited. However, there is recent compelling evidence that yeast have functional alternative splicing, mainly in response to environmental conditions. We have previously shown that sequence and structure properties of the pre-mRNA could explain the selection of 3' splice sites (ss) in Saccharomyces cerevisiae. In this work, we extend our previous observations to build a computational classifier that explains most of the annotated 3'ss in the CDS and 5' UTR of this organism. Moreover, we show that the same rules can explain the selection of alternative 3'ss. Experimental validation of a number of predicted alternative 3'ss shows that their usage is low compared to annotated 3'ss. The majority of these alternative 3'ss introduce premature termination codons (PTCs), suggesting a role in expression regulation. Furthermore, a genome-wide analysis of the effect of temperature, followed by experimental validation, yields only a small number of changes, indicating that this type of regulation is not widespread. Our results are consistent with the presence of alternative 3'ss selection in yeast mediated by the pre-mRNA structure, which can be responsive to external cues, like temperature, and is possibly related to the control of gene expression.

Abstract
Graphs are a very important abstraction to model complex structures and respective interactions, with a broad range of applications including web analysis, telecommunications, chemical informatics and bioinformatics. In this work we are interested in the application of graph mining to identify abnormal behavior patterns from telecom Call Detail Records (CDRs). Such behaviors could also be used to model essential business tasks in telecom, for example churning, fraud, or marketing strategies, where the number of customers is typically quite large. Therefore, it is important to rank the most interesting patterns for further analysis. We propose a vertex relevant ranking score as a unified measure for focusing the search of abnormal patterns in weighted call graphs based on CDRs. Classical graph-vertex measures usually expose a quantitative perspective of vertices in telecom call graphs. We aggregate wellknown vertex measures for handling attribute-based information usually provided by CDRs. Experimental evaluation carried out with real data streams, from a local mobile telecom company, showed us the feasibility of the proposed strategy. © 2012 Springer-Verlag.

Abstract
In this paper the integer-valued autoregressive model of order one, contaminated with additive outliers is studied in some detail. Moreover, parameter estimation is also addressed. Supposing that the timepoints of the outliers are known but their sizes are unknown, we prove that the conditional least squares (CLS) estimators of the offspring and innovation means are strongly consistent. In contrast, however, the CLS estimators of the outliers' sizes are not strongly consistent, although they converge to a random limit with probability 1. We also prove that the joint CLS estimator of the offspring and innovation means is asymptotically normal. Conditionally on the values of the process at the timepoints neighboring to the outliers' occurrences, the joint CLS estimator of the sizes of the outliers is also asymptotically normal.

Abstract
In the last decades propofol became established as an intravenous agent for the induction and maintenance of both sedation and general anesthesia procedures. In order to achieve the desired clinical effects appropriate infusion rate strategies must be designed. Moreover, it is important to avoid or minimize side effects which may be associated with adverse cardiorespiratory effects and delayed recovery. Nowadays, to attain these purposes the continuous propofol delivery is usually performed through target-controlled infusion (TCI) systems whose algorithms rely on pharmacokinetic and pharmacodynamic models (Schraag, 2001). This work presents statistical models to estimate both the infusion rate and the bolus administration. The modeling strategy relies on multivariate linear models for panel data (Wooldridge, 2002), based on patient characteristics such as age, height, weight and gender along with the desired target concentration. A clinical database collected with a RugLoopII device on 84 patients undergoing ultrasonographic endoscopy under sedation-analgesia with propofol and remifentanil, (Gambús et al., 2011), is used to estimate the models (training set with 74 cases) and assess their performance (test set with 10 cases). The results obtained in the test set comprising a broad range of characteristics are satisfactory since the models are able to predict bolus and infusion rates comparable to those of TCI. © 2012 IFAC.

Abstract
In this paper we focus on the real-time frequency domain analysis of speech signals, and on the extraction of suitable and perceptually meaningful features that are related to the glottal source and that may pave the way for robust speaker identification and voice register classification. We take advantage of an analysis-synthesis framework derived from an audio coding algorithm in order to estimate and model the relative delays between the different harmonics reflecting the contribution of the glottal source and the group delay of the vocal tract filter. We show in this paper that this approach effectively captures the shape invariance of a periodic signal and may be suited to monitor and extract in real-time perceptually important features correlating well with specific voice registers or with a speaker unique sound signature. A first validation study is described that confirms the competitive performance of the proposed approach in the automatic classification of the breathy, normal and pressed voice phonation types.