Abstract

Abstract
Long Period Fibre Gratings (LPFGs) were fabricated by femtosecond (fs) laser direct writing in a standard single-mode fibre (SMF-28e) to measure variations in the surrounding refractive index (SRI). The sensing sensitivity of these structures was optimized with the deposition of homogeneous thin layers of titanium dioxide (TiO2) by physical vapour deposition (PVD) process. A set of LPFGs were coated with different thickness layers of TiO2, and the spectral features were monitored for different SRI solutions. The wavelength shift and the optical power variation of the LPFG minimum attenuation band were measured achieving sensitivities of similar to 570 nm/RIU at using SRI near to 1.3600 in the case of the LPFG coated with 60 nm of TiO2, a 10-fold increase over the corresponding for a bare LPFG. For SRI values higher than the cladding refractive index, a sensitivity over similar to 3000 nm/RIU was determined for 30 nm of TiO2 thick film, a region where the bare LPFGs are useless. For 30 nm of TiO2, the optical power variation follows a quasi-linear function of the SRI, with a range of similar to 10 dB. Moreover, values as high as 50 and 120 dB/RIU at 1.3200 and 1.4200, respectively, can be obtained by choosing the proper film thickness. Preliminary studies revealed that coating fs-laser direct writing LPFGs with titanium dioxide improves their performance.

Abstract
Long period fiber gratings (LPFGs) were fabricated in a standard single mode fiber (SMF-28e) through femtosecond (fs) laser direct writing. LPFGs with longer and shorter periods were fabricated, which allows coupling from the fundamental core mode to lower and higher order asymmetric cladding modes (LP(1,6)and LP1,12, respectively). For the grating periods of 182.7 and 192.5 mu m, it was verified that the LP(1,12)mode exhibits a TAP at approximately 1380 and 1448 nm in air and water, respectively. Characterization of the LPFGs subjected to high-temperature thermal treatment was accomplished. Fine-tuning of the resonance band's position and thermal stability up to 600 degrees C was shown. The temperature sensitivity was characterized for the gratings with different periods and for different temperature ranges. A maximum sensitivity of -180.73, and 179.29 pm/degrees C was obtained for the two resonances of the 182.7 mu m TAP LPFG, in the range between 250 and 600 degrees C.

Abstract
Biogenic amines, such as putrescine are potential indicators of food storage condition and deterioration. The real time measurement of their concentration in food may become an important method of food control. It was found that putrescine diffuses through a thin layer made from a solution of Poly(ethylene-co-vinyl acetate) (PEVA) and maleic anhydride. Poly(ethylene-co-vinyl acetate) is a common non-chlorinated vinyl capable to adsorb specific analytes as putrescine which upon diffusion, reversibly binds to the maleic anhydride causing the polymer swelling resulting in spectral changes from the optical point of view. Long Period Fiber Gratings coated with 30 nm titanium dioxide, a high refractive index material used to increase the intrinsic sensitivity to the external refractive index, were overcoated with a thin layer of maleic anhydride doped Poly(ethylene-co-vinyl acetate). When exposed to solutions containing small concentrations of putrescine the resonant band corresponding to the LP1,6 cladding mode was found to move to shorter wavelengths. The observed blue shift corresponds to the increasing concentration of putrescine in the fiber sensor structure. Further work is being carried out to improve the sensitivity and the limit of detection of the sensing system as well as to increase range of operation, which is presently limited to 0.3 to 0.5 M.

Abstract
This study aims to evaluate the effect of Salvia officinalis L. (sage) essential oil (EO) on behavior of L. monocytogenes ATCC679 inoculated in beef processed by Sous-vide cook-chill (SVCC) and stored at 2 or 8 degrees C during 28 days. Minimum inhibitory concentration (MIC) of L. monocytogenes was obtained with 31.3 mu L/mL of EO. D values were determined for samples with EO (21'39 '') and without EO (21'17 ''). Beef samples were inoculated with L. monocytogenes at a concentration of 1 x 108 CFU/mL and vacuum-packed after EO addition at MIC value. Three heat treatments (F) were applied to reduce 1-log10 (F1), 2-log10 (F2) and 3-log10 (F3). EO composition was identified by gas-chromatography mass-spectrometry analysis. The main compounds identified were beta-pinene (11.70%), camphor (8.21%), beta-thujene (7.82%), 1.8-cineole (5.19%), alpha-humulene (6.07%) and endoborneol (4.87%).A reduction of approximately 1 log (CFU/g) of L. monocytogenes was observed in EO samples, compared to control samples at 2 degrees C. At 8 degrees C, despite exponential development from day 14, lower L. monocytogenes counts were observed in EO samples. Data showed that sage EO can help to control L. monocytogenes growth. However a possibility of using sage as a natural preservative, must be combined with other agents to control microbial growth more effectively.

Abstract
Biogenic amines (BAs) are well-known biomolecules, mostly for their toxic and carcinogenic effects. Commonly, they are used as an indicator of quality preservation in food and beverages since their presence in higher concentrations is associated with poor quality. With respect to BA's metabolic pathways, time plays a crucial factor in their formation. They are mainly formed by microbial decarboxylation of amino acids, which is closely related to food deterioration, therefore, making them unfit for human consumption. Pathogenic microorganisms grow in food without any noticeable change in odor, appearance, or taste, thus, they can reach toxic concentrations. The present review provides an overview of the most recent literature on BAs with special emphasis on food matrixes, including a description of the typical BA assay formats, along with its general structure, according to the biorecognition elements used (enzymes, nucleic acids, whole cells, and antibodies). The extensive and significant amount of research that has been done to the investigation of biorecognition elements, transducers, and their integration in biosensors, over the years has been reviewed.