Abstract
Sensing at the single cell level can provide insights into its dynamics and heterogeneity, yielding information otherwise unattainable with traditional biological methods where average population behavior is observed. In this context, optical tweezers provide the ability to select, separate, manipulate and identify single cells or other types of microparticles, potentially enabling single cell diagnostics. Forward or backscatter analysis of the light interacting with the trapped cells can provide valuable insights on the cell optical, geometrical and mechanical properties. In particular, the combination of tweezers systems with advanced machine learning algorithms can enable single cell identification capabilities. However, typical processing pipelines require a training stage which often struggles when trying to generalize to new sets of data. In this context, fully automated tweezers system can provide mechanisms to obtain much larger datasets with minimum effort form the users, while eliminating procedural variability. In this work, a pipeline for full automation of optical tweezers systems is discussed. A performance comparison between manually operated and fully automated tweezers systems is presented, clearly showing advantages of the latter. A case study demonstrating the ability of the system to discriminate molecular binding events on microparticles is presented.

Abstract
Exploring optical analogues with paraxial fluids of light has been a subject of great interest over the past years. Despite many optical analogues having been created and explored with these systems, they have some limitations that usually hinder the observation of the desired dynamics. Since these systems map the effective time onto the propagation direction, the fixed size of the nonlinear media limits the experimental effective time, and only the output state is accessible. In this work, we present a solution to overcome these problems in the form of an optical feedback loop, which consists of reconstructing the output state, by using the off-axis digital holography technique, and then re-injecting it again at the entrance of the medium through the utilization of Spatial Light Modulators. This technique enables access to intermediate states and an extension of the system effective time. Furthermore, the total control of the amplitude and phase of the beam at the input of the medium, also allows us to explore more exotic configurations that may be interesting in the context of optical analogues, that otherwise would be hard to create. To demonstrate the capabilities of the setup, we explore qualitatively some case studies, such as the dark soliton decay into vortices with the propagation of shock waves, and the collision dynamics between three flat-top states. The results presented in this work pave the way for probing new dynamics with paraxial fluids of light.