Abstract
This paper reports the use of different generations of polyamidoamine (PAMAM) dendrimers for the in vitro transfection of mesenchymal stem cells (MSCs). A systematic study was carried out on the transfection efficiency achieved by the PAMAM dendrimers using a p-galactosidase reporter gene system. Transfection results were shown to be dependent upon the generation of dendrimers, the amine to phosphate group ratio and the cell passage number. In all cases, the transfection efficiency was very low. Nevertheless, it was hypothesized that a low transfection level could be sufficient to promote the in vitro differentiation of MSCs towards the osteoblastic lineage. To address this possibility, dendrimers carrying the human bone morphogenetic protein-2 (hBMP-2) gene-containing plasmid were used. All quantitative (alkaline phosphatase activity, osteocalcin secretion and calcium deposition) and qualitative (von Kossa staining) osteogenic markers were significantly stronger in transfected cells when compared to non-transfected ones. This study not only clearly demonstrates that a low transfection level can be sufficient for inducing in vitro differentiation of MSCs to the osteoblast phenotype but also highlights the importance of focusing research on the development of gene delivery vectors in the concrete application.

Abstract
A long-period grating written in the SMF-28 fibre was heat treated at 1000 degrees C for 15 days. The spectrum of the grating shifted to longer wavelengths and the resonances depth decreased as a result of structural relaxation. The background loss increased considerably for times longer than 200 h, and this loss is caused by devitrification of the fibre.

Abstract
A novel miniature fiber Bragg grating-based temperature probe is presented. The sensor design integrates a u-shape lossless taper, thus offering the advantages of a terminal temperature probe while enabling effective serial multiplexing. We report on the experimental validation of the temperature probe design, demonstrating lossless operation and effective elimination of strain cross-sensitivity.

Abstract
A passive all-fibre technique for Bragg wavelength shift detection is presented which can be considered as the minimum configuration for fibre grating sensing demodulation. The scheme involves a light source with a particular spectral profile that allows the direct tracking of Bragg wavelength shifts. Referencing to source power fluctuations is also provided. This simple technique shows satisfactory resolution over a large dynamic range. Experimental results are presented for temperature and strain measurements. © 1996 IOP Publishing Ltd.

Abstract
A fiber optic sensor for liquid refractive index measurement based on a Fabry-Perot interferometer is described. The interferometer is achieved between the reflection of a short fiber Bragg grating and the Fresnel reflection from the cleaved fiber end. This fiber end is then in contact with the liquid sample to provide refractive index measurements. The sensor is characterized by immersing the fiber tip in distilled water with different concentrations of ethylene glycol. A linear relation of the interferometer fringe visibility with refractive index variation is observed, and a resolution of similar to 10(-3) is obtained. It is also shown that the sensor operation is independent of temperature effects, other than the one related to temperature-induced change of the liquid refractive index. (c) 2008 Society of Photo-Optical Instrumentation Engineers.

Abstract
We present results on the characterization of the response of a dual resonance observed in the spectrum of a single long-period grating arc-induced in a B/Ge co-doped fiber to different physical parameters. The dual resonance is formed by two overlapping resonances corresponding to coupling of the core mode to symmetric and antisymmetric cladding modes. The behavior of the resonances is studied when the grating is subject to strain, bending, torsion, temperature, or external refractive-index changes. The strain, bending, and torsion sensitivities of the two resonances differ, whereas the temperature sensitivities are almost the same. The sensitivities to variation in external refractive index are the same for the two resonances when the long-period grating is straight and differ when the fiber with the grating is curved. (c) 2010 Optical Society of America