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Publicações

Publicações por CRAS

2014

DNA-Aptamer optical biosensors based on a LPG-SPR optical fiber platform for point-of care diagnostic

Autores
Coelho, L; Queiros, RB; Santos, JL; Martins, MCL; Viegas, D; Jorge, PAS;

Publicação
PLASMONICS IN BIOLOGY AND MEDICINE XI

Abstract
Surface Plasmon Resonance (SPR) is the base for some of the most sensitive label free optical fiber biosensors. However, most solutions presented to date require the use of fragile fiber optic structure such as adiabatic tapers or side polished fibers. On the other hand, long-period fiber gratings (LPG) present themselves as an interesting solution to attain an evanescent wave refractive index sensor platform while preserving the optical fiber integrity. The combination of these two approaches constitute a powerful platform that can potentially reach the highest sensitivities as it was recently demonstrated by detailed theoretical study [1, 2]. In this work, a LPG-SPR platform is explored in different configurations (metal coating between two LPG - symmetric and asymmetric) operating in the telecom band (around 1550 nm). For this purpose LPGs with period of 396 mu m are combined with tailor made metallic thin films. In particular, the sensing regions were coated with 2 nm of chromium to improve the adhesion to the fiber and 16 nm of gold followed by a 100 nm thick layer of TiO2 dielectric material strategically chosen to attain plasmon resonance in the desired wavelength range. The obtained refractometric platforms were then validated as a biosensor. For this purpose the detection of thrombin using an aptamer based probe was used as a model system for protein detection. The surface of the sensing fibers were cleaned with isopropanol and dried with N-2 and then the aminated thrombin aptamer (5'-[NH2]-GGTTGGTGTGGTTGG-3') was immobilized by physisorption using Poly-L-Lysine (PLL) as cationic polymer. Preliminary results indicate the viability of the LPFG-SPR-APTAMER as a flexible platforms point of care diagnostic biosensors.

2014

Enhanced refractive index sensing characteristics of optical fibre long period grating coated with titanium dioxide thin films

Autores
Coelho, L; Viegas, D; Santos, JL; de Almeida, JMMM;

Publicação
SENSORS AND ACTUATORS B-CHEMICAL

Abstract
A new type of fibre-optic refractive index sensor based on a long period fibre grating (LPFG) coated with a titanium dioxide (TiO2) thin film was demonstrated. The wavelength shift of the attenuation bands of this LPFG sensor to changes in the refractive index of the external media from 1.30 to 1.64 RIU was investigated. In order to optimize the sensor the TiO2 thin film thickness deposited around the LPFGs was varied from 10 to 80 nm. It was found that the TiO2 thin film increases the wavelength sensitivity of the LPFG to changes in the surrounding refractive index for values lower and higher than the cladding refractive index. As opposed to the bare LPFG it was shown the possibility to monitor refractive indices lower and higher than cladding refractive index tailoring the TiO2 thickness. An average wavelength sensitivity of 5250 nm/RIU was achieved in the range 1.444 to 1.456 RIU for a TiO2 thickness of 50 nm. In the region between 1.46 and 1.48 RIU the average sensitivity of about 825 nm/RIU was measured for a 40 nm thick film.

2014

Optical sensing based in plasmonics and the metamaterials enhancement factor

Autores
Moayyed, H; Leite, IT; Coelho, L; Santos, JL; Guerreiro, A; Viegas, D;

Publicação
Latin America Optics and Photonics Conference, LAOP 2014

Abstract
The recent burst of R&D activity in Plasmonics, associated with the possibility of materials nanostructuring which enables the access to metamaterials, has been strongly impacting many branches of optics such as imaging, data recording and sensing. This talk details the factors that turned the combination Plasmonics and Metamaterials a huge opportunity to optical sensing.© OSA 2014.

2014

Analysis of Phase Interrogated SPR Fiber Optic Sensors With Bimetallic Layers

Autores
Moayyed, H; Leite, IT; Coelho, L; Santos, JL; Viegas, D;

Publicação
IEEE SENSORS JOURNAL

Abstract
An effective analytical model combining geometrical optics with the transfer-matrix theory for stratified optical media is applied to investigate the sensing properties of tapered optical fiber surface plasmon resonance (SPR) sensors incorporating Ag-Au bimetallic layers, particularly in the context of phase interrogation. The performance of the sensing structures is studied as a function of the tapering parameters and thickness of the metallic layers. It is shown that the Ag-Au bimetallic combination is capable of improving the resolution and tuning working region of SPR fiber-optic sensors and that by tapering the sensing structures enhanced sensitivity can be achieved when phase interrogation is considered.

2014

Analysis of Phase Interrogated SPR Fiber Optic Sensors with Different Bimetallic Combinations

Autores
Moayyed, H; Leite, IT; Coelho, L; Santos, JL; Guerreiro, A; Viegas, D;

Publicação
SECOND INTERNATIONAL CONFERENCE ON APPLICATIONS OF OPTICS AND PHOTONICS

Abstract
Optical fiber sensors based on the phenomenon of plasmonic resonance can be interrogated applying different methods, the most common one being the spectral approach where the measurand information is derived from the reading of the wavelength resonance dip. In principle, a far better performance can be achieved considering the reading of the phase of the light at a specific wavelength located within the spectral plasmonic resonance. This approach is investigated in this work for surface plasmon based fiber optic sensors with overlays which are combinations of bimetallic layers, permitting not only to tune the wavelength of the plasmon resonance but also the sensitivity associated with the phase interrogation of the sensors. The metals considered for the present analysis are silver, gold, copper, and aluminum.

2014

Rock strength assessment and structural features analysis on rocky coasts

Autores
Pires, A; Chamine, HI; Perez Alberti, A; Gomes, A; Rocha, F;

Publicação
ROCK ENGINEERING AND ROCK MECHANICS: STRUCTURES IN AND ON ROCK MASSES

Abstract
This work addresses the rock matrix strength comparative analysis on rocky coasts. A coastal geoengineering integrated system for rocky coast assessment was proposed and applied in two fissured granitic key-sites in NW Iberia (NW Portugal, and Galicia). The main stages of the study comprise six steps: (i) high-resolution aerial imagery survey; (ii) visual inspection and systematic monitoring; (iii) applied field datasheet; (iv) in situ evaluation; (v) scanline survey; (vi) coastal geosciences engineering GIS based mapping. The Schmidt Hammer and Equotip tester were applied to measure the rebound and hardness, respectively, in rocky platforms, geoforms and coastal boulders. Moreover a detailed description of discontinuities and a geologic-geotechnical evaluation in the rocky platforms was performed. The current rock project is of great interest especially to highly dynamic coastal areas. This approach evolving geomorphic processes, geoengineering issues and geomechanical testing assessment contributes to support coastal management/planning.

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